P2Y₁₂ regulates platelet adhesion/activation, thrombus growth, and thrombus stability in injured arteries
J. Clin. Invest. Patrick André, et al. 112:398 doi:10.1172/JCI17864 [Go to this article.]

Figure 8
Flow-cytometric analysis of Alexa 488-fibrinogen binding to resting platelets, 0.6 mM mTRAP-treated platelets, and 5 μM ADP-treated platelets, in the presence or absence of 10 μM epinephrine-activated (Epi) and ADP plus epinephrine–activated platelets. Thick line represents P2Y₁₂^–/– platelets; thin line WT platelets. Epinephrine (1 and 10 μM) did not induce binding of Alexa 488-fibrinogen in absence of 1 mM CaCl₂ (not shown). The presence of epinephrine in ADP- and mTRAP-activated P2Y₁₂^–/– platelets gave a pattern similar to that of WT platelets treated with the same agonist in the absence of epinephrine. Data shown are representative of at least three experiments performed in duplicate.