P2Y₁₂ regulates platelet adhesion/activation, thrombus growth, and thrombus stability in injured arteries
J. Clin. Invest. Patrick André, et al. 112:398 doi:10.1172/JCI17864 [Go to this article.]

Figure 1
Targeting the P2Y₁₂ locus. (a) The neomycin resistance gene in pLNL is flanked by a 4-kb (5′) and 6-kb (3′) fragment from the P2Y₁₂ genomic locus. HindIII, XbaI, XhoI, and NotI sites were introduced by PCR. The WT P2Y₁₂ coding exon is denoted as ORF. ORF, open reading frame.(b) Southern hybridizations of XbaI-digested mouse genomic DNA from progeny of breeding pairs heterozygous at the mutant allele. The XbaI fragment detected by the indicated probe (gray oval in Figure 1a) is reduced from 9.1 to 8.3 kb due to introduction of a new XbaI site in the targeted locus. Intro, introduction.