Bone marrow stem cells contribute to repair of the ischemically injured renal tubule
J. Clin. Invest. Sujata Kale, et al. 112:42 doi:10.1172/JCI17856 [Go to this article.]

Figure 6
BMA worsens the course of ischemic ATN. (a) BUN values from mice that underwent bilateral ischemia for 30 minutes followed by 7 days of recovery. Mice underwent BMA prior to I/R (BMA); underwent BMA prior to I/R and stem cell transplantation after I/R (BMA-SCT); or underwent I/R without BMA (control). (b and c) X-gal staining of kidney sections reveals multiple β-galactosidase–positive tubules in the kidneys of BMA-SCT animals 48 hours after I/R (b) and 7 days after I/R (c). Of note, the sections in b were not counterstained with neutral fast red. (d–g) H&E staining reveals tubular damage in the outer medulla of both groups, with extensive denudation of some of the tubular basement membranes in animals that underwent BMA alone (d and e, arrowheads). The majority of damaged tubules in the BMA-SCT group contain flattened cells lining the basement membrane (g, arrows), while other areas demonstrate almost complete resolution of the injury (f). ×40.