Selective stimulation of VEGFR-1 prevents oxygen-induced retinal vascular degeneration in retinopathy of prematurity
J. Clin. Invest. Shu-Ching Shih, et al. 112:50 doi:10.1172/JCI17808 [Go to this article.]

Figure 4
Immunohistochemical localization of VEGFR-1 and VEGFR-2 protein in P5 and P15 retinal cross sections. (a–f) P5 immunohistochemical localization of (a) VEGFR-1, (d) VEGFR-2, (b and e) G. simplicifolia I isolectin–stained endothelial cells, and (c and f) merged images. (a) VEGFR-1 protein (green) is seen primarily in the ganglion cell layer (GCL) and is seen to overlap with (b) endothelial cells (red) when (c) the images are merged (yellow; indicated by arrows). (d) VEGFR-2–positive signal (green) is found in the GCL, the inner plexiform layer, the inner nuclear layer (INL), and the outer nuclear layer (ONL). (e) Vascular endothelial cells (red) do not overlap with VEGFR-2–positive cells in the merged image (f) and are not coincident with vessels (arrows). (g–l) P15 immunohistochemical localization of (g) VEGFR-1 and (j) VEGFR-2 staining and (h and k) isolectin-stained endothelial cells. (i and l) Merged images. VEGFR-1 protein (green in g) completely overlaps with endothelial cells (red in h) when the images are merged (yellow in i). Some VEGFR-2 signal (green in j) overlaps with endothelial cells (k) when the images are merged (yellow in l), whereas other VEGFR-2–positive cells (indicated by arrow in d and f) span the retina and are morphologically consistent with Muller cell structure. RPE/Ch, retinal pigment epithelium/choroid.