Selective stimulation of VEGFR-1 prevents oxygen-induced retinal vascular degeneration in retinopathy of prematurity
J. Clin. Invest. Shu-Ching Shih, et al. 112:50 doi:10.1172/JCI17808 [Go to this article.]

Figure 3
Immunohistochemical localization of VEGFR-1 and VEGFR-2 protein in P2 and P5 retinal whole mounts. On P5, VEGFR-1 protein (red) is detected in a vascular pattern (a and c) and clearly coincides with endothelial cells (blue) as detected with G. simplicifolia I isolectin (d). (e) A merged image (purple) shows coincidence. (b and f) VEGFR-2–positive signal (red) is found in the neural retina, specifically in the interstices between vessels (blue) (g) in the merged image (h), which shows little or no overlap between vessels and VEGFR-2 staining. On P2, VEGFR-1 protein signal (green) (i) coincides with endothelial cells (red) (j) detected by lectin. In a merged image (k), retinal vessels coincide with the faint VEGFR-1 staining. The retinal vessel front is defined by an arrow. Some hyaloid vasculature (H) from incomplete removal on the vitreal surface of the P2 retina is also positive for VEGFR-1 signal. (l) VEGFR-2–positive signal (green) is found in the neural retina and does not coincide with endothelial capillaries (red) (m) in the merged image (n). Arrows indicate the vessel front.