Inefficient establishment of KSHV latency suggests an additional role for continued lytic replication in Kaposi sarcoma pathogenesis
J. Clin. Invest. Adam Grundhoff, et al. 113:124 doi:10.1172/JCI17803 [Go to this article.]

Figure 3
Efficient establishment of artificial KSHV episomes in vitro requires antibiotic selection and expression of LANA in trans. SLK₇₃ cells were transfected with pGFP (open circles/dashed lines), pGTR4 (open squares/solid lines), or pGTR8 (filled squares/solid lines), and the transfected cultures were maintained in the presence (a) or absence (b) of G418. Cultures were analyzed at the indicated time points after transfection by FACS (left panels in a and b) or Hirt-PCR (right panels).