AIP1 mediates TNF-α–induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3
J. Clin. Invest. Rong Zhang, et al. 111:1933 doi:10.1172/JCI17790 [Go to this article.]

Figure 4
TNF-α disrupts intramolecular interaction between the N-terminus and C-terminus of AIP1. (a) AIP1 folds in a closed form. BAECs were transfected with AIP1-F and AIP1-C, followed by treatment with 10 ng/ml TNF-α for 15 minutes (+) or no treatment (–). Cell lysates were immunoprecipitated with either anti–AIP1-N or anti–AIP1-C of AIP1, followed by Western blot with anti-FLAG. (b) TNF-α disrupts interaction of AIP1-C and AIP1-N. ECs were transfected with AIP1-C and AIP1-N, followed by TNF-α treatment as in a (+). Expression of AIP1-C and AIP1-N was detected by Western blot with anti-FLAG. Cell lysates were immunoprecipitated with anti–AIP1-N, followed by Western blot with anti–AIP1-C (lanes 3–4).