DNA vaccination with CD25 protects rats from adjuvant arthritis and induces an antiergotypic response
J. Clin. Invest. Avishai Mimran, et al. 113:924 doi:10.1172/JCI17772 [
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Figure 6The antiergotypic T cell proliferative response following DNA vaccination. Ten days after DNA vaccination, DLN cells of three rats per group were pooled from (A) empty vector–vaccinated rats (MSP = 229 cpm) or (B) from CD25-vaccinated rats (MSP = 164 cpm), and the T cell responses to irradiated A6-S or A6-R were measured. The test was repeated at day 22 after AA induction in (C) rats vaccinated with the empty vector (MSP = 198 cpm) or (D) with the CD25 gene (MSP = 223 cpm). Stimulator cells were used at the indicated doses. Proliferative responses are presented as the SI ± SEM of quadruplicate cultures. This is a representative experiment of three repetitions. *
P < 0.01 compared with the CD25-protected rats (D).
