DNA vaccination with CD25 protects rats from adjuvant arthritis and induces an antiergotypic response
J. Clin. Invest. Avishai Mimran, et al. 113:924 doi:10.1172/JCI17772 [
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Figure 4Adoptive transfer of antiergotypic T cells protects from AA. Antiergotypic T cells were obtained from the DLN of rats vaccinated with irradiated, activated, syngeneic A2b T cells. Control cells were obtained from rats vaccinated with resting A2b T cells. The antiergotypic LN cells and control LN cells were stimulated in vitro with activated or resting D9 T cells, respectively. Naive rats were injected with 10 × 10
6 antiergotypic T cells or control T cells. Ten days later the rats were boosted with 5 × 10
6 cells, and AA was induced on the same day (day 0). AA scores were assessed every 2 days starting at day 12. The mean ± SEM disease score is shown. Scores of the antiergotypic T cell–vaccinated group were significantly reduced compared with the control T cell–vaccinated group for each of the days 14–26 (
P < 0.01). The
P value of day 26 is indicated.
