Inhibition of growth hormone action improves insulin sensitivity in liver IGF-1–deficient mice
J. Clin. Invest. Shoshana Yakar, et al. 113:96 doi:10.1172/JCI17763 [Go to this article.]

Figure 1
Comparison of IGF-1 levels, body weight, and organ weight in control (C), LID, GHa, and LID + GHa mice. (a) Serum was obtained as described in Methods and treated with acid-ethanol to remove IGFBPs. Total IGF-1 levels were determined by RIA in serum samples from control mice (n = 15), LID mice (n = 21), GHa mice (n = 12), and LID + GHa mice (n = 14). *P < 0.01 compared with control mice; **P < 0.01 compared with LID or GHa mice. (b) Body weight was measured at weekly intervals from birth to the age of 12 weeks (n = 15–30 mice per group). (c) Organ weight was measured at 16–18 weeks of age and is expressed as the average percentage of body weight. Data are expressed as average ± SE. (d) Total body fat content was measured using dual-energy x-ray absorptiometry analysis of at least five mice per group. Data are expressed as average ± SE. *P < 0.01 compared with control mice. (e) Serum leptin was determined by RIA in serum samples from control mice (n = 8), LID mice (n = 8), GHa mice (n = 10), and LID + GHa mice (n = 8). *P < 0.01 compared with control mice; **P < 0.01 compared with LID mice.