Recipient-type specific CD4⁺CD25⁺ regulatory T cells favor immune reconstitution and control graft-versus-host disease while maintaining graft-versus-leukemia
J. Clin. Invest. Aurélie Trenado, et al. 112:1688 doi:10.1172/JCI17702 [Go to this article.]

Figure 1
Regulation of GVHD by the addition of ex vivo–expanded Treg’s. At the end of the culture, Treg’s were tested for their capacity to control GVHD in the BALB/c → [BALB/c × C3H]F1 combination. (a) The picture illustrates the skin lesions and general status of grafted [BALB/c × C3H]F1 mice undergoing GVHD (upper pairs) or mice protected from GVHD by adding sTreg’s (lower pairs). (b) Mice were weighed at different time points prior to sacrifice at day 45. Mean weight curves were established for mice receiving BM cells alone (dashed line, n = 3), BM cells supplemented with 10 × 10⁶ conventional T cells (open circles, n = 5) in addition to either 10 × 10⁶ sTreg’s (filled squares, n = 15) or irTreg’s (filled circles, n = 14). P < 0.05 between all groups except for BM cells alone versus sTreg’s. (c) Histopathologic score of liver and spleen after semiallogeneic BMT. Grading of GVHD was performed 45 days after transplantation in liver and spleen. BM control mice infused with BM cells alone did not develop GVHD (n = 3). ND, not done. GVHD control mice received BM cells plus T cells and represented the maximum intensity of GVHD in this model (n = 4). Experimental mice received BM cells plus T cells and either sTreg’s (n = 8) or irTreg’s (n = 7). Points correspond to histopathological scores of individual mice; histograms show the mean histopathological score for each group. P < 0.05 between all groups for all tissues except for BM cells alone versus sTreg’s and BM cells alone versus irTreg’s in the liver.