Inhibition of endogenous thioredoxin in the heart increases oxidative stress and cardiac hypertrophy
J. Clin. Invest. Mitsutaka Yamamoto, et al. 112:1395 doi:10.1172/JCI17700 [
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Figure 5Tg-DN-Trx1 mice and NTg littermates were subjected to either transverse aortic banding or sham operation. (
a) The effect of pressure overload on expression of endogenous mouse Trx1 in NTg mice. The result is representative of four experiments. (
b–
d) Aortic banding was applied for 2 weeks. (
b) The effect of pressure overload on oxidative stress in the heart. The heart content of MDA and of MDA plus 4-HAE, the percentage increase in MDA, and the percentage increase in MDA plus 4-HAE are shown. (
c and
d) LVW/BW (
c) and percentage increase in LVW/BW in response to aortic banding (
d) were determined. Similar results were obtained for LVW/TL. The percentage increase in LVW/BW and LVW/TL (not shown) was significantly greater in Tg-DN-Trx1 than in NTg mice in response to pressure overload. (
e and
f) Aortic banding was applied for 4 weeks. LVW/BW (
e) and percentage increase in LVW/BW in response to aortic banding (
f) were determined. The percentage increase in LVW/BW was significantly greater in Tg-DN-Trx1 than in NTg mice.
