Expansion of human SCID-repopulating cells under hypoxic conditions
J. Clin. Invest. Guénahel H. Danet, et al. 112:126 doi:10.1172/JCI17669 [Go to this article.]

Figure 4
Expression of HIF in BM progenitors and stem cells. (a) RT-PCR analysis of ARNT and HIF-1α performed on RNA extracted from BM Lin^–CD34⁺CD38^–, Lin^–CD34^–CD38^–, and K562 cells (as a positive control). (b) Western blot analysis of cell extracts from BM Lin^–CD34⁺ cells cultured overnight under hypoxic (H) or normoxic (N) conditions using antibodies against ARNT and HIF-1α. All gels are representative of at least three experiments.