Expansion of human SCID-repopulating cells under hypoxic conditions
J. Clin. Invest. Guénahel H. Danet, et al. 112:126 doi:10.1172/JCI17669 [
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Figure 3Quantitative analysis of the effect of hypoxia on SRC frequency. The frequency of SRCs in freshly isolated Lin
–CD34
+CD38
– cells (
a, day 0) or cells cultured for 4 days under normoxic (
b) or hypoxic (1.5% O
2) (
c) conditions was determined by limiting dilution analysis. For each NOD/SCID mouse (
n = 88), the level of human engraftment (percentage of CD45
+ cells) is represented as a function of the number of Lin
–CD34
+CD38
– cells transplanted. (
d) Representative flow cytometry dot plot showing the presence of both human myeloid (CD33
+) and lymphoid (CD19
+) cells in NOD/SCID BM 6–8 weeks after transplantation.
