Expansion of human SCID-repopulating cells under hypoxic conditions
J. Clin. Invest. Guénahel H. Danet, et al. 112:126 doi:10.1172/JCI17669 [Go to this article.]

Figure 1
Expansion and division history of BM cells after 4 days in culture in hypoxia or normoxia. Lin^–CD34⁺ and Lin^–CD34⁺CD38^– cells were cultured in serum-free conditions for 4 days in the presence of IL-3, IL-6, SCF, Flt-3 ligand, and G-CSF. (a) Expansion. The fold increase in cell number relative to the initial cell number plated (day 0) is represented for each subpopulation cultured in normoxia (white bars) or hypoxia (black bars). *P < 0.05. (b) Division history of primitive subpopulations of Lin^–CD34⁺ cells. Freshly isolated Lin^–CD34⁺ cells were labeled with CFSE, cultured for 4 days in hypoxia (1.5% O₂) or normoxia, and analyzed for CFSE fluorescence intensity and expression of markers associated with primitive progenitors and stem cells. Histograms (representative of three separate experiments) of CFSE fluorescence in various Lin^–CD34⁺ cell subsets are shown after 4 days of culture in hypoxia (solid lines) or normoxia (dashed lines). Day 0 CFSE fluorescence intensity is indicated by an arrow on each histogram.