JunD protects against chronic kidney disease by regulating paracrine mitogens
J. Clin. Invest. Evangéline Pillebout, et al. 112:843 doi:10.1172/JCI17647 [
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Figure 4Elevated TGF-α expression in the absence of JunD. (
a) EGF and TGF-α mRNA expression in control nonoperated (C) and remnant (Nx) kidneys of
JunD+/+ (white bars) and
JunD–/– (black bars) mice at day 60. Data are mean ± SEM;
n = 6 for each point. *
P < 0.05, **
P < 0.01, remnant versus control kidneys;
###P < 0.001,
JunD–/– versus
JunD+/+ kidneys by ANOVA, followed by Tukey-Kramer test. (
b) Immunohistochemistry of EGF (upper) and TGF-α (lower) expression in control nonoperated and remnant (Nx) kidneys of
JunD+/+ and
JunD–/– mice at day 60 (×200). (
c) Immunoblot analysis of TGF-α expression in control nonoperated (C) and remnant (Nx) kidneys of
JunD+/+ and
JunD–/– mice at day 60. Extracts of
TGF-α–/– kidneys served as negative controls.
