JunD protects against chronic kidney disease by regulating paracrine mitogens
J. Clin. Invest. Evangéline Pillebout, et al. 112:843 doi:10.1172/JCI17647 [
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Figure 3Increased tubular cell proliferation in the absence of JunD. (
a) The number of PCNA-positive cells per tubular section was measured in control kidneys (gray bar) and in
JunD+/+ (white bars) and
JunD–/– (black bars) remnant kidneys 7, 14, 30, and 60 days after nephron reduction. Data are mean ± SEM;
n = 4–6 for each time point. ***
P < 0.001, remnant versus control kidneys;
###P < 0.001,
JunD–/– versus
JunD+/+ by ANOVA, followed by Tukey-Kramer test. (
b) PCNA and β-gal immunostaining in serial 4-μm sections of
JunD–/– remnant kidneys at day 60 (×200). (
c) Colocalization experiments in serial sections of
JunD–/– remnant kidneys at day 60. Upper panels: overlay of β-gal staining and LTL staining (left) or Tamm-Horsfall staining (right). Lower panels: overlay of PCNA staining and LTL staining (left) or Tamm-Horsfall staining (right). (×200).
