Memory CD4⁺ T cells do not induce graft-versus-host disease
J. Clin. Invest. Britt E. Anderson, et al. 112:101 doi:10.1172/JCI17601 [Go to this article.]

Figure 4
AutoMACS- and FACS-sorted CD25-depleted memory T cells do not cause GVHD. Donor B10.D2 spleen cells enriched for CD4⁺ T cells using BioMag beads were stained with biotinylated anti-CD62L and anti-CD25 mAb’s, followed by staining with SA-beads. Cells were separated into CD25^–CD62L^– (negative [neg] fraction) and CD25⁺CD62L⁺ (positive [pos] fraction) cells using an AutoMACS. Phenotype of presort CD4⁺ T cells is shown in (a). Phenotype of CD25^–CD62L^– negative fraction (memory cells) is shown in (b). CD25⁺CD62L⁺ cells (positive fraction) were sorted on a FACStar cell sorter to purify CD25^– (c) and CD62L⁺CD44^– cells (d). Reanalysis of the sorted population is not available. BALB/c mice were lethally irradiated and reconstituted with 8 × 10⁶ B10.D2 T cell–depleted BM alone (thin dashed line, n = 5) or with 1.5 × 10⁶ unfractionated B10.D2 CD4⁺ T cells (thin solid line, n = 10), 2.5 × 10⁵ CD4⁺CD25^– naive T cells (thick solid line, n = 5), or 10⁶ CD4⁺CD25^– memory T cells (thick dashed line, n = 4). Incidence of GVHD (e). P < 0.0002 and P < 0.003 for difference between recipients of CD25^– memory and total CD4 and CD25^– naive CD4 cells, respectively. Average clinical disease score for mice affected with GVHD (f). *P < 0.02 (all time points) for CD25^– memory versus total CD4. P < 0.01 on days 19–43 after transplant for recipients of CD25^– memory versus naive CD4 cells. Pathology scoring from representative mice (g). ^#P < 0.007 and P < 0.014 for recipients of CD25^– memory versus total and CD25^– naive CD4 cells, respectively.