Memory CD4⁺ T cells do not induce graft-versus-host disease
J. Clin. Invest. Britt E. Anderson, et al. 112:101 doi:10.1172/JCI17601 [Go to this article.]

Figure 3
FACS-sorted memory CD25^– T cells do not cause GVHD. Donor B10.D2 spleen cells were enriched for CD4⁺ T cells using BioMag separation, then stained with mAb’s for CD4, CD25, CD62L, and CD44. After gating on CD4⁺CD25^– cells (a), T cells were sorted on the basis of CD62L and CD44 expression (b). Reanalyses of sorted populations are shown in (c) (CD44 versus CD62L) and (d) (CD4 versus CD25). BALB/c mice were lethally irradiated and reconstituted with 8 × 10⁶ B10.D2 T cell–depleted BM alone (thin dashed line, n = 1) or with 2 × 10⁶ B10.D2 unfractionated CD4⁺ T cells (thin solid line, n = 4), 10⁶ purified naive CD4⁺CD25^– T cells (thick solid line, n = 9), or 10⁶ memory CD4⁺CD25^– T cells (thick dashed line, n = 3). Incidence of GVHD is shown in (e). P < 0.0082 and P < 0.0005 comparing GVHD incidence in recipients of CD25^– memory CD4 versus unfractionated and CD25^– naive CD4 cells, respectively. Average clinical disease score for affected mice (f). *P < 0.05 (time points 1–3) and for recipients of CD25^– naive cells versus unfractionated CD4 cells. P < 0.02 for all comparisons between recipients of CD25^– memory and naive cells. Pathology scoring from representative mice (g). ^††P < 0.0034 and P < 0.017 for recipients of memory versus total and naive CD4⁺ T cells, respectively. **P < 0.016 for recipients of naive versus total CD4⁺ T cells.