Memory CD4⁺ T cells do not induce graft-versus-host disease
J. Clin. Invest. Britt E. Anderson, et al. 112:101 doi:10.1172/JCI17601 [Go to this article.]

Figure 1
Memory CD4⁺ T cells do not cause GVHD. Naive and memory T cells were purified as described in Methods. After gating on CD4⁺ T cells (a), cells were sorted into CD62L⁺CD44^– naive and CD62L^–CD44⁺ memory fractions (b). Reanalyses of sorted populations are shown in (c). BALB/c mice were lethally irradiated and reconstituted with 8 × 10⁶ B10.D2 T cell–depleted BM alone (thin dashed line, n = 9) or with 10⁷ B10.D2 total spleen cells (thin solid line, n = 25), 10⁶ naive T cells (thick solid line, n = 20), or 10⁶ memory T cells (thick dashed line, n = 10). Data are combined from two independent experiments. GVHD incidence and mean clinical score are shown in d and e. Statistical comparisons are as follows: (d). P < 0.0001 for GVHD incidence in recipients of memory CD4 versus spleen cells or naive CD4 cells. (e) For clinical score, *P < 0.05 (time points 1–3) and ^‡P < 0.01 (time points 4–6) for recipients of naive versus total spleen cells; ^†P < 0.05. ^§P < 0.001 (time points 2–10) for recipients of memory versus total spleen cells. P < 0.0001 for recipients of memory versus naive cells at all time points. BM control mice and BM plus memory cell groups did not get GVHD, but the clinical score lines were offset for clarity. Pathology scores from representative mice are shown in (f). Mean scores are indicated by horizontal bars. ^††P < 0.005 and P < 0.0004 for recipients of memory versus total (unfractionated) spleen cells and memory versus naive CD4 cells, respectively.