Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair
J. Clin. Invest. Myriam Alcalay, et al. 112:1751 doi:10.1172/JCI17595 [Go to this article.]

Figure 2
(a) Semiquantitative PCR analysis of 14 predicted targets. For each gene, the number of cycles required to detect a signal in the linear range was previously determined (see “Supplementary Methods,” ref.12). (b) Semiquantitative PCR analysis of AML blasts compared to CD34⁺ normal precursors of 11 targets identified in the U937 system. Relative mRNA levels of induced target genes (c) and of repressed target genes (d) in U937 PML/RAR, U937 AML1/ETO or U937 PLZF/RAR cells assessed by real time RT-PCR. Mt was the control used in panels a, c, and d. (e) Expression levels of common target genes in U937 PML/RAR cells before and after 4 or 8 hours of treatment with 10^–6 M RA. Values are calculated relatively to expression levels in Mt cells receiving the same treatment. (f) Expression levels of common target genes in blasts derived form two APL patients (APL no. 1 and APL no. 2) after 4 hours of in vitro treatment with 10^–6 M RA. All values are shown as compared to the control (C); i.e., expression levels in blasts from the same individual, prior to RA treatment. All experiments shown in this figure were performed in triplicate. One representative experiment is shown for semiquantiative PCRs (a and b), whereas an average value of the three results was plotted for real time RT-PCR data (c–f). GAPDH gene expression was used to normalize all experiments.