Critical role of the Toll-like receptor signal adaptor protein MyD88 in acute allograft rejection
J. Clin. Invest. Daniel R. Goldstein, et al. 111:1571 doi:10.1172/JCI17573 [Go to this article.]

Figure 5
Impaired Th1 immune but intact Th2 immune responses in MyD88^–/– recipients of male allografts. WT and mutant mice were grafted with corresponding male allografts, and draining lymph nodes were isolated 14 days after transplantation. Gene expression for IFN-γ (a) and IL-4 (b) was quantified using real-time PCR and reported as fold increase compared with that of naive mice in each group. The results show that in comparison to WT counterparts, MyD88^–/– recipients had impaired upregulation of IFN-γ gene expression (5.5-fold vs. 23-fold increase, *P = 0.0002). However, IL-4 gene expression was equally well expressed (4.0-fold vs. 3.14-fold increase, **P = 0.6). Experiments were repeated in triplicate.