Critical role of the Toll-like receptor signal adaptor protein MyD88 in acute allograft rejection
J. Clin. Invest. Daniel R. Goldstein, et al. 111:1571 doi:10.1172/JCI17573 [Go to this article.]

Figure 1
(a) Rejection of HY-incompatible skin allografts depends on the presence of MyD88. Eight out of nine MyD88^–/– female recipients were unable to reject MyD88^–/– male donor skin grafts (filled circles), whereas WT littermate controls (filled squares) manifested a median allograft survival time of 16 days (P = 0.00001). TLR2^–/– (triangles) and ICE^–/– (open squares) recipients demonstrated a modest but significant delay in allograft rejection (P = 0.02 and P = 0.01, respectively), whereas allograft rejection in TLR4^–/– recipients (diamonds) was not different from that in controls (P = 0.13) despite the presence of one outlier that did not reject its allograft. (b) Adoptive transfer of primed WT spleen cells restores allograft rejection. MyD88^–/– female recipients infused in vivo with 4 × 10⁷ primed WT spleen cells at the time of transplantation (squares) rejected their HY-incompatible allografts. P = 0.00003 vs. a control group (diamonds) of female MyD88^–/– recipients that were infused with primed male MyD88^–/– spleen cells.