Mature CD8⁺ T lymphocyte response to viral infection during fetal life
J. Clin. Invest. Arnaud Marchant, et al. 111:1747 doi:10.1172/JCI17470 [Go to this article.]

Figure 2
TCR BV spectratypic analysis of CD8⁺ T lymphocytes and phenotypic analysis of BV-specific CD8⁺ T cells from control and HCMV-infected newborns. (a) Spectratypic analysis. CDR3 length distribution was Gaussian for all BV families of control newborn no. 9 (shows five representative families), indicating a naive CD8⁺ T cell repertoire. Similar results were obtained in control newborns no. 8 and 12. In contrast, alterations in CDR3 length distribution were detected in BV16, BV18, and BV23 of case no. 7, indicating oligoclonal expansions of CD8⁺ T cells. (b and c) Phenotypic analysis. Expression of HLA-DR, CD27, and CD28 by BV2, BV14, BV16, BV18, and BV23 CD8⁺ T cells of control no. 9 and case no. 7. High proportions of CD8⁺ T lymphocytes from HCMV-infected newborns had a phenotype of activated (b) and differentiated (c) cells. These alterations were primarily observed in the BV families that included oligoclonal expansions in the spectratypic analysis (BV16, BV18, and BV23).