Constitutive p40 promoter activation and IL-23 production in the terminal ileum mediated by dendritic cells
J. Clin. Invest. Christoph Becker, et al. 112:693 doi:10.1172/JCI17464 [Go to this article.]

Figure 6
LPDCs are differentially located in the small intestine as compared with the colon and are largely CD11b^–CD8α^–. Location of LPDCs in the colon and small bowel. Cryosections were analyzed by immunofluorescence using the tyramide signal amplification Cy3 or FITC system and fluorescence microscopy. Cryosections were stained for CD11c, IL-12 p40, CD11b, and CD8a, as indicated. Nuclei were counterstained in blue. (a) Differential localization of LPDCs in the colon (upper panels) and small intestine (lower panels). The lamina propria of the small bowel contained many CD11c⁺ LPDCs as well as some CD11c⁺ subepithelial cells, whereas few LPDCs were seen in the colon. (b) Staining for CD11b⁺ (red), CD11c⁺ (green), and CD11b⁺ plus CD11c⁺ (yellow) cells in the distal small bowel of healthy mice, showing that most CD11c⁺ LPDCs do not coexpress CD11b. (c) Staining for CD11c (green), CD8α (red), and CD11c plus CD8α (yellow) cells in the distal small bowel (D4) of healthy FVB mice (upper panels), showing that most CD11c⁺ LPDCs do not coexpress CD8a. CD11c⁺ LPDCs did express MHC class II molecules, as determined by immunohistochemistry, however (not shown). No staining for CD8α was noted in T cell–deficient RAG knockout mice (RAG^–/–; lower panels), although many CD11c⁺; LPDCs were detected in these animals.