Constitutive p40 promoter activation and IL-23 production in the terminal ileum mediated by dendritic cells
J. Clin. Invest. Christoph Becker, et al. 112:693 doi:10.1172/JCI17464 [Go to this article.]

Figure 3
Analysis of various organs from IL-12 p40 promoter/luciferase transgenic mice. (a) Luciferase activity in different tissues of untreated, healthy transgenic mice. Luciferase expression was measured in a standard luminometer after homogenization of whole organs from transgenic mice. Results were normalized to the protein content of the homogenates and are presented as relative light units (RLU) per milligram of protein extract ± SD of five independent experiments. All founder lines of IL-12 p40 promoter/luciferase transgenic mice showed the highest constitutive activity in the small intestine, whereas little activity was seen in the spleen, liver, kidney, heart, and colon. (b) Luciferase activity in different segments of the small intestine from untreated, healthy transgenic mice upon removal of the Peyer’s patches. The small intestine was divided into four segments of equal length (from the proximal segment D1 to the distal segment D4), and luciferase expression was measured in a standard luminometer after homogenization of gut samples from transgenic mice. Results were normalized to the protein content of the homogenates and are presented as relative light units per milligram of protein extract ± SD of three independent experiments. All founder lines of IL-12 p40 promoter/luciferase transgenic mice showed the highest constitutive activity in the distal segment of the small intestine, whereas little activity was seen in proximal segments.