Constitutive p40 promoter activation and IL-23 production in the terminal ileum mediated by dendritic cells
J. Clin. Invest. Christoph Becker, et al. 112:693 doi:10.1172/JCI17464 [Go to this article.]

Figure 2
Cell-specific and stimulation-dependent expression of the transgene. (a) Upper panel: Luciferase expression in the spleen of control and LPS-injected transgenic mice. Mice were injected intraperitoneally with 200 μg of LPS. After 4 hours, splenic luciferase expression was assessed. Data are shown as mean values of three mice per group ± SD. LPS injection led to increased luciferase activity indicating LPS-dependent p40 promoter activity in spleen cells. Lower panel: IL-12 p40 protein levels in the spleen of the same animals as above were determined by ELISA analysis of splenic cell lysates. Mean values ± SD are shown. (b) Luciferase expression in splenic macrophages, T cells, and B cells of transgenic mice upon stimulation with LPS/SAC (macrophages, B cells) or anti-CD3 plus anti-CD28 Ab’s (T cells) for 24 hours. Date represent mean values ± SD of three mice per group. (c) Luciferase activity in peritoneal macrophages of transgenic mice. Cells were isolated as described in Methods and stimulated for 24 hours as indicated. The data represent mean values ± SD of three independent experiments.