Constitutive p40 promoter activation and IL-23 production in the terminal ileum mediated by dendritic cells
J. Clin. Invest. Christoph Becker, et al. 112:693 doi:10.1172/JCI17464 [Go to this article.]

Figure 1
Generation of IL-12 p40 promoter/luciferase transgenic mice. (a) Reporter gene analysis of the p40/pXP1 reporter gene construct in various cell lines. The IL-12 p40 promoter was cloned as a Stul restriction enzyme fragment upstream of the luciferase reporter gene into the pXP1 vector yielding the p40/pXP1 vector. P40/pXP1 was transfected in various cell lines using the DEAE transfection method. Cells were left untreated or were stimulated for 8 hours with PMA/ionomycin or LPS/IFN-γ, as indicated. Data represent average values of two independent experiments and are presented as fold induction of relative light units (RLU) as compared with the transfection of the empty pXP1 reporter gene vector. (b and c) Map of the luciferase expression cassette and generation of IL-12 p40 promoter/luciferase transgenic mice. A 4.7-kb IL-12 p40 promoter/luciferase expression cassette was used for the generation of transgenic animals. The screening of transgenic mice was performed by isolation of tail DNA and subsequent PCR analysis with a set of construct-specific primers, giving rise to a single band of 1,081 bp. Several founder mice were identified by PCR that were used for subsequent analysis.