Bone marrow transplantation reveals an essential synergy between neuronal and hemopoietic cell neurokinin production in pulmonary inflammation
J. Clin. Invest. Mara Chavolla-Calderón, et al. 111:973 doi:10.1172/JCI17458 [Go to this article.]

Figure 4
(a–c) Immunostaining of lung tissue for substance P after immune complex–mediated injury. Substance P immunoreactivity (IR, red arrows) was detected only in macrophages (but not other inflammatory cells) of WT mice reconstituted with WT bone marrow (a) and PPT-A gene–deleted mice reconstituted with WT bone marrow (b). No substance P immunoreactivity was present in macrophages (green arrows) or other cells in PPT-A gene–deleted mice (c). (d) Decreased substance P immunoreactivity (IR), by ELISA, in the bronchoalveolar fluid of WT, PPT-A gene–deleted (PPT-A^–/–) mice reconstituted with WT bone marrow, WT mice reconstituted with PPT-A^–/– bone marrow, and capsaicin-pretreated mice, after an immune complex–mediated lung injury (P = 0.03). Comparisons are relative to WT mice reconstituted with WT bone marrow, which serve as a WT control for the effects of conditioning irradiation. Values are shown as mean ± SE (n = 5 for all groups).