Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization
J. Clin. Invest. Tatsuya Kondo, et al. 111:1835 doi:10.1172/JCI17455 [Go to this article.]

Figure 2
Retinas of VENIRKO and VENIFARKO mice exposed to hypoxia show fewer neovascular nuclei on PAS and hematoxylin staining. Frozen sections (6 μm) of retinas were stained using PAS and hematoxylin. Retinas prepared from each control (a and e), VENIRKO (b), and VENIFARKO (f) mouse exposed in normoxia showed no neovascularization. Sections from each control mouse in the ΔO₂ model (c and g) had significant numbers of protruding nuclei above the inner limiting membrane, whereas the number of nuclei in VENIRKO retinas after the same treatment (d) was decreased 57% as compared with controls (P < 0.01; see Figure 6a). Furthermore, the number of nuclei in VENIFARKO retinas (h) was reduced 34%, as in control mice (P < 0.05; see Figure 6a).