Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization
J. Clin. Invest. Tatsuya Kondo, et al. 111:1835 doi:10.1172/JCI17455 [Go to this article.]

Figure 1
Retinal flat-mount pictures of VENIRKO and VENIFARKO mice exposed to hypoxia show decreased loss of blood vessels and fewer vascular tufts. Controls (b and f), VENIRKO (c and g), and VENIFARKO (d and h) mice were placed in a 75% oxygen environment from P7 to P12, then returned to normoxia for 5 days. Control in normoxia sample is shown in a and e. Retinal vessels were visualized by fluorescein-conjugated dextran injection at P17. Control in normoxia shows no central avascular area (a) and no peripheral tuft formation (e). Low magnification of retina from control mice exposed to hypoxia (b) shows large central avascular areas (indicated by arrows). High magnifications of these retinas (f) reveal neovascular tuft formation (arrowheads). Retinas of VENIRKO mice (c and g) show smaller avascular areas and a reduction of tufts. Retinas of VENIFARKO mice (d and h) show intermediate central avascular areas and tufts between control and VENIRKO mice. These flat-mount pictures are representative of at least six similar experiments.