WNK kinases regulate thiazide-sensitive Na-Cl cotransport
J. Clin. Invest. Chao-Ling Yang, et al. 111:1039 doi:10.1172/JCI17443 [Go to this article.]

Figure 1
(a) Effect of WNK4 on NCC-mediated ²²Na uptake, expressed as nmol/h per oocyte. Uptake rates ± SEM of oocytes injected with cRNA encoding NCC, WNK4, NCC + WNK4, NCC + an antisense WNK4 (AS WNK4), NCC + SGK (an unrelated kinase), and water are shown. WNK4 alone has no effect on uptake. Coexpression with WNK4 significantly reduces NCC-mediated Na uptake. Neither antisense WNK4 nor SGK affects NCC activity. *Uptake significantly different from NCC injection alone; P < 0.001. (b) Immunoblot of oocytes probed with anti-WNK4 antibody: oocytes were injected with water or with cRNA encoding WNK4, WNK4 + NCC, or NCC alone. Only oocytes injected with WNK4 cRNA express WNK4 protein. For NCC, WNK4, and NCC + WNK4, there were 12 repetitions of each experiment (15–30 oocytes per experiment). For NCC + antisense WNK4 and NCC + SGK, there were four experiments.Relative molecular weights are shown in kDa. HCTZ, hydrochlorothiazide.