Group B streptococcal β-hemolysin/cytolysin activates neutrophil signaling pathways in brain endothelium and contributes to development of meningitis
J. Clin. Invest. Kelly S. Doran, et al. 112:736 doi:10.1172/JCI17335 [Go to this article.]

Figure 1
Effect of GBS mutations on transcript levels. We used real-time RT-PCR to verify the differential expression for five transcripts (IL-8, Groβ, Groα, IL-6, and GM-CSF) after infection with WT, β-h/c–deficient (β-h/c^–), or capsule-deficient (cps^–) GBS. The levels of IL-8, IL-6, Groα, and GM-CSF message were normalized to the 18S rRNA standard and Groβ to GAPDH. Representative data from duplicate experiments are shown. The mean of triplicate wells resulted in SDs of less than ± 1.5 in all cases. *P < 0.05 compared with WT. **P < 0.001 compared with WT.