A functional androgen receptor is not sufficient to allow estradiol to protect bone after gonadectomy in estradiol receptor–deficient mice
J. Clin. Invest. Natalie A. Sims, et al. 111:1319 doi:10.1172/JCI17246 [Go to this article.]

Figure 3
Testosterone prevents orchidectomy-induced bone loss in WT and ERα^–/– males. Antiandrogen treatment partially reproduced the effects of orchidectomy. (a) Representative von Kossa–stained sections of the proximal tibia from WT (left) and ERα^–/– (right) males that were sham-operated (Sham), orchidectomized (Orx), orchidectomized and treated with 10 mg/kg/d subcutaneous propiotestosterone (Orx+T), or sham-operated and treated subcutaneously with the antiandrogen RU58642 (30 mg/kg/d) (Sham+aA). (b) BV/TV and Tb.BMD were protected from bone loss after orchidectomy by testosterone treatment. Antiandrogen treatment partially reproduced the bone loss observed after orchidectomy when administered to sham-operated males of both genotypes. (c) The increased ObS/BS, BFR/BS, and OcS/BS after orchidectomy were prevented by testosterone treatment in both WT and ERα^–/– males. Antiandrogen treatment increased these markers of bone turnover in sham-operated males of both genotypes. Values are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham-operated of the same genotype; ⁺P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 vs. Orx of the same genotype.