CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells
J. Clin. Invest. Eric J. Kunkel, et al. 111:1001 doi:10.1172/JCI17244 [Go to this article.]

Figure 2
CCR10 is expressed on mucosal tissue lymphocytes with a PC phenotype. Lymphocytes isolated from the gastrointestinal tract or tonsil were stained for CD19, CD20, and CD38 to define various B cell subsets (gated on large lymphocytes by scatter). (a) Both the colon and tonsil contained significant populations of both GC (CD38⁺CD19⁺CD20^hi) and PC (CD38^hiCD19^+/–CD20^–) phenotype B cells. GC cells did not express either CCR10 or CCR9 (<3% of GC cells in all tissues examined), while a fraction of PCs in the tonsil (22% ± 4%) and virtually all PCs in the colon (92% ± 4%) expressed CCR10. (b) CCR10 was also expressed on the vast majority of PCs in the jejunum (90% ± 7%), ileum (92% ± 5%), and stomach (87% ± 10%), and a significant fraction of cells in the appendix (38% ± 11%). CCR9 was present on PCs in the jejunum (36% ± 14%) and ileum (41% ± 18%) where TECK is also expressed and on PCs in the stomach (13% ± 7%). (c) GC cells in both the tonsil and colon expressed high levels of CD19 (>95% positive), while the phenotypically defined PC in both tissues had largely downregulated CD19 (<15% positive). Flow-cytometry data are representative of two tonsil, three stomach, three jejunum, three ileum, three colon, and two appendix samples with mean ± SD shown. Percentage of CCR10- or CCR9-positive cells based on quadrant encompassing 5% of isotype control-stained cells in dot blots.