Intestinal heat shock protein 110 regulates expression of CD1d on intestinal epithelial cells
J. Clin. Invest. Sean P. Colgan, et al. 112:745 doi:10.1172/JCI17241 [Go to this article.]

Figure 4
Non–microbe-derived factors are present within the lumen of the murine small and large intestine that upregulate CD1d expression on human T84 cells. Cell surface ELISA was performed on confluent T84 monolayers using the CD1d-specific antibody 51.1.3. (a) BALB/c mice housed in either germfree (GF) or SPF environments were sacrificed and the LC of the colon isolated. T84 cells were cultured alone (–) or exposed to the soluble lumenal preparations for 48 hours at a final concentration of 6 μg/ml. (b) Lumenal material from the stomach (St), small intestine (SI), or large intestine (LI) of SPF-housed mice was tested for its ability to enhance epithelial cell surface CD1d expression. The CD1d-inducing activity of both soluble lumenal components (black bars) and mucosal scrapings (gray bars) was assessed relative to untreated controls (–).