Intestinal heat shock protein 110 regulates expression of CD1d on intestinal epithelial cells
J. Clin. Invest. Sean P. Colgan, et al. 112:745 doi:10.1172/JCI17241 [Go to this article.]

Figure 3
Soluble LC material elevates CD1d expression in the colonic epithelium-derived cell lines HT-29 and Caco-2. (a) CD1d-specific cell surface ELISA of confluent monolayers of HT-29 cells following incubation with LC samples. As a positive control, cells were exposed to IFN-γ at a final concentration of 1,000 IU/ml for a period of 48 hours. (b) Western blot of whole-cell lysates isolated from HT-29 and Caco-2 cells following treatment with soluble LC material at a concentration of 6 μg/ml. Proteins contained within the lysates were resolved by 12% SDS-PAGE and transferred to nitrocellulose. The presence of CD1d was subsequently assessed by probing with the anti-human CD1d-specific antibody D5. CD1d was observed as dominant bands of 48-kDa (filled arrowhead) and 37-kDa (open arrowhead) forms. No bands were detected when nitrocellulose membranes were probed with normal mouse serum (data not shown). The blots were reprobed for the presence of β-actin (bottom panels).