Role of RANK ligand in mediating increased bone resorption in early postmenopausal women
J. Clin. Invest. Guitty Eghbali-Fatourechi, et al. 111:1221 doi:10.1172/JCI17215 [Go to this article.]

Figure 3
Demonstration of the lack of TRAIL expression in cells sorted by FACS using RT-PCR analysis. In ethidium bromide–stained agarose gel of PCR products, BAP⁺/RANKL^– cells sorted by dual-color flow cytometry are shown in lane 1 and BAP⁺/RANKL⁺ cells are shown in lane 2. Size markers are given in lane 3. Note that the BAP⁺ cells binding the OPG-Fc-FITC probe (lane 2) express the RANKL, but not TRAIL mRNA. Conversely, the BAP⁺ cells not binding the OPG-Fc-FITC probe (lane 1) express TRAIL mRNA, but do not express RANKL.