Toll-like receptor-dependent production of IL-12p40 causes chronic enterocolitis in myeloid cell-specific Stat3-deficient mice
J. Clin. Invest. Masaya Kobayashi, et al. 111:1297 doi:10.1172/JCI17085 [Go to this article.]

Figure 3
IBDs in TNF-α/Stat3 double-deficient mice. (a–d) Histologic examination of the colons of (a) control, (b) TNF-α–deficient, (c) Stat3 mutant, and (d) TNF-α/Stat3 double-mutant mice at 16 weeks. H&E staining is shown. ×20. (e) The colitis score of TNF-α/Stat3 double-mutant mice at 16 weeks of age. TNF-α/Stat3 double-mutant mice developed severe chronic colitis, similar to Stat3 mutant mice. *P < 0.001 versus group of control mice. (f) Mice were intraperitoneally injected with thioglycollate, and 3 days later peritoneal macrophages were isolated. Macrophages were stimulated with 10 ng/ml LPS for 24 h. Concentrations of TNF-α, IL-6, and IL-12p40 in the culture supernatants were measured. *Not detected.