Altered blood pressure responses and normal cardiac phenotype in ACE2-null mice
J. Clin. Invest. Susan B. Gurley, et al. 116:2218 doi:10.1172/JCI16980 [Go to this article.]

Figure 1
Generation of ACE2 KO mice. (A) Strategy for producing targeted disruption of the Ace2 gene. In the targeting vector, the exon containing nucleotides +1069 to +1299 encoding the active site of the ACE2 enzyme (including the Zn-binding signature motif, HEMGH) was replaced with a NEO/URA3 cassette. (B) The genotype at the Ace2 locus was determined by Southern blot hybridization using the 5′ flanking probe. After digestion of genomic DNA by EcoRI, a 6-kb band was diagnostic of the targeted allele, and the WT allele was identified by an 11-kb band. Het, heterozygous. (C) Northern blot analysis of kidneys from Ace2^–/y and Ace2^+/y mice hybridized with a cDNA probe for mouse ACE2. ACE2 mRNA was not detected in kidney RNA from Ace2^–/y mice.