Neuropeptide Y induces ischemic angiogenesis and restores function of ischemic skeletal muscles
J. Clin. Invest. Edward W. Lee, et al. 111:1853 doi:10.1172/JCI16929 [
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Figure 2NPY stimulates capillary angiogenesis (
a and
b) and collateral formation (
c) in rat ischemic hindlimb. (
a) Density of vWF- and cd31-positive (a measure of total microvascular density), and latex-filled (a measure of vessels perfused, red arrowheads) vessels in ischemic and nonischemic gastrocnemius muscles (×100). Angiogenic index is a ratio of latex-perfused to total microvascular density. (
b) Density of vWF-positive vessels in ischemic and nonischemic tissues with and without NPY, Y1 receptor antagonist (active and inactive), and placebo (**
P < 0.01 compared with ischemic and placebo group [white bars,
n=10]. (
c) Representative vessels in the area around the femoral artery occlusion in the adductor muscle (bars = 20 μm) following perfusion with latex (H&E staining) and graph showing number of these arteries/mm
2 (**
P < 0.01,
n = 10). Arrows indicate media thickness. Ant, antagonist.
