Osteopetrosis and thalamic hypomyelinosis with synaptic degeneration in DAP12-deficient mice
J. Clin. Invest. Tomonori Kaifu, et al. 111:323 doi:10.1172/JCI16923 [
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Figure 4Thalamus-accentuated hypomyelinosis in
DAP12–/– mice. (
a) Thalamus-accentuated reduction of MBP staining. Coronal brain sections were stained with anti-MBP antibody. The left side is from
DAP12–/– mouse and the right side is from wild-type mouse, both at 3 months of age. Each panel is composed of multiple pictures. Arrow indicates the thalamus-accentuated reduction of MBP staining. fm, fimbria; ic, internal capsule; ptm, putamen; thlm, thalamus. Scale bar, 250 μm. (
b–
e) Ultrastructure of medial thalamus (
b–
d) and putamen (
e). (
b) Lower-magnification views (original magnification: ×2,000) of medial thalamus of 6-month-old
DAP12–/– and wild-type mice. Myelinated axons are reduced significantly in the
DAP12–/– mouse, while blood vessels appear to be normal (arrow). (
c and
d) Higher-magnification views (×10,000 for
c, ×50,000 for
d) showing many unmyelinated axons with aberrant morphology (arrows) and electron-dense appearance of the non-axonal area (
c), and synaptic degeneration and abnormal accumulation of synaptic vesicles (flanked by arrowheads) in the
DAP12–/– mouse (
d). (
e) Putamen of 3-month-old mice. Hypomyelinosis is not evident in this area, but a somewhat distorted axon shape is evident. Original magnification: ×40,000. Scale bar, 250 nm.
