IL-12 is required for differentiation of pathogenic CD8⁺ T cell effectors that cause myocarditis
J. Clin. Invest. Nir Grabie, et al. 111:671 doi:10.1172/JCI16867 [Go to this article.]

Figure 5
In vivo migration and proliferation of OT-I effector cells. (a) Hematoxylin- and eosin-stained sections were prepared from hearts harvested at 48 hours after T cell transfer of 2.5 × 10⁶ OT-I⁰ or OT-I^IL-12 effector cells. (b) Enlarged peribronchial lymph nodes, consistently found in the illustrated location in CMy-mOva mice with myocarditis, were harvested 48 hours after adoptive transfer of 2.5 × 10⁶ OT-I⁰ or OT^-I^IL-12 effectors, and FACS analysis was performed as described in Methods. (c) CFSE-labeled OT-I effector cells were transferred into CMy-mOva mice, and peribronchial lymph nodes were harvested after 72 hours for flow cytometric analysis. The histograms show CFSE fluorescence intensity in Thy 1.1⁺–gated cells. The horizontal bars indicate the range of fluorescence intensity detected in effector cells immediately after labeling, before transfer. (d) The histological grade of myocarditis was assessed, as described in Methods, in hearts harvested at various times after adoptive transfer of 3 × 10⁶ OT-I effectors.