IL-12 is required for differentiation of pathogenic CD8⁺ T cell effectors that cause myocarditis
J. Clin. Invest. Nir Grabie, et al. 111:671 doi:10.1172/JCI16867 [Go to this article.]

Figure 3
OT-I T-cell–mediated myocarditis in CMy-mOva mice. (a) Mice were injected with indicated numbers of OT-I⁰ or OT-I^IL-12 effector T cells, whose phenotypes are described in Figure 2, or with OT-I^IL-12 cells differentiated in the presence of C57BL/6 STAT-4^–/– splenic APCs. Mean survival is shown for each group. Data represent means ± SD (n = 4 per group). Additional studies with more than 16 mice confirmed that CMy-mOva mice survived longer than 72 days after adoptive transfer of 5 × 10⁶ OT-I⁰. (b) Hearts were harvested 72 hours after adoptive transfer of 10⁶ OT-I effector T cell populations (described in Figure 2). Low- and high-power photomicrographs of hematoxylin- and eosin-stained sections and immunohistochemical stains for CD90.1 (Thy 1.1), which specifically marks the OT-I cells, are shown.