A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I
J. Clin. Invest. Nan Wang, et al. 111:99 doi:10.1172/JCI16808 [
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Figure 5Effects of cholesterol and phospholipid efflux on the induction of ABCA1 by apoA-I. (
a, top) ABCA1 protein level in mouse peritoneal macrophages after 3 hours of incubation with 10 μg/ml apoA-I or 50 μg/ml HDL
2, or after pretreatment with 1 mM methyl-β-cyclodextrin (MβCD) for 5 minutes to deplete cholesterol and then with control media for 3 hours. (
a, bottom) Phospholipid (Pl) and cholesterol (Ch) efflux in macrophages treatments similar to those in the top panel. (
b) ABCA1-FLAG and Walker-mutant (K939M) ABCA1-FLAG (ABCA1WM) protein levels after 3 hours of incubation with apoA-I (10 μg/ml) in transiently transfected 293 cells. (
c) Wild-type ABCA1-FLAG and ABCA1-W590S-FLAG protein levels after 3 hours of incubation with apoA-I (10 μg/ml) or calpeptin (20 μg/ml) in 293 cells. The data are representative of two (
a and
b) or three (
c) experiments with similar results.
