A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I
J. Clin. Invest. Nan Wang, et al. 111:99 doi:10.1172/JCI16808 [Go to this article.]

Figure 4
ApoA-I–mediated increase in ABCA1 requires PEST sequence. (a) Dose-response effect of apoA-I (3 hours’ incubation) in HEK293 cells transfected with wild-type ABCA1. (b) Wild-type ABCA1 and ABCA1delPEST protein levels after 3 hours of incubation with apoA-I (10 μg/ml) in 293 cells transiently transfected with the FLAG-tagged constructs, as determined by Western blot with FLAG antibodies. The data are representative of three (a) or two (b) experiments with similar results.