Keratinocyte growth factor and the transcription factors C/EBPα, C/EBPδ, and SREBP-1c regulate fatty acid synthesis in alveolar type II cells
J. Clin. Invest. Robert J. Mason, et al. 112:244 doi:10.1172/JCI16793 [Go to this article.]

Figure 4
Fatty acid synthesis is stimulated by the LXR agonist T0901317. Type II cells were plated on Matrigel in 5% RS and then cultured in 1% CS-FBS with or without KGF and with or without varying doses of T0901317 and appropriate vehicle controls. (a) T0901317 increased acetate incorporation in a dose-dependent manner in 1% CS-FBS and at the highest dose in the presence of KGF. The results are normalized to the value of 1% CS-FBS and are means ± SEM for five independent experiments. *P < 0.05 vs. the appropriate DMSO control without the LXR agonist. (b) Protein expression is shown by immunoblotting. The conditions are the same as in a. Lanes 1, 2, 7, and 8 contain DMSO without T0901317 as shown in a. Protein loading is normalized to the amount of actin. A representative sample of five independent experiments is shown.