Keratinocyte growth factor and the transcription factors C/EBPα, C/EBPδ, and SREBP-1c regulate fatty acid synthesis in alveolar type II cells
J. Clin. Invest. Robert J. Mason, et al. 112:244 doi:10.1172/JCI16793 [
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Figure 3C/EBPα, C/EBPδ, and SREBP-1 protein levels are increased in the differentiated cultures. Type II cells were cultured in 1% CS-FBS alone (lane 1), plus KGF (lane 2), plus 5% RS (lane 3), or plus 5% RS and KGF (lane 4) for 6 days in the apical-access system under air/liquid conditions. The cells were extracted and the protein expression measured by Western analysis as described in Methods. Because matrix proteins within the gel precluded a reliable measurement of cellular protein, loading of individual lanes was based on the amount of actin. A representative example of at least three determinations is shown.
