Keratinocyte growth factor and the transcription factors C/EBPα, C/EBPδ, and SREBP-1c regulate fatty acid synthesis in alveolar type II cells
J. Clin. Invest. Robert J. Mason, et al. 112:244 doi:10.1172/JCI16793 [Go to this article.]

Figure 2
C/EBPα, SCD-1, and SCD-2 mRNA are increased in type II cells in response to KGF. (a–l) Type II cells were cultured in 5% RS with or without KGF for 6 days in the apical-access system. The monolayers were fixed with 4% paraformaldehyde, processed for in situ hybridization with sense and antisense probes, and stained with hematoxylin. In a (bright field) and c (dark field), the results for RS alone are shown. The expression is very low but higher than with the sense probe (data not shown). In b (bright field) and d (dark field), there is increased expression of C/EBPα in the presence of RS plus KGF. Similarly, KGF stimulates the expression of SCD-1 (e–h) and SCD-2 (i–l). (m and n) The expression of SCD-1 is shown in the normal lung in bright field (m) and dark field (n). Specific cells in the alveolar region are heavily labeled, and the distribution of these cells is the same as that of SP-C, a marker restricted to type II cells. The micrographs were taken at ×400.