Lipoprotein lipase (LpL) on the surface of cardiomyocytes increases lipid uptake and produces a cardiomyopathy
J. Clin. Invest. Hiroaki Yagyu, et al. 111:419 doi:10.1172/JCI16751 [Go to this article.]

Figure 4
VLDL turnover studies in hLpL^GPI/LpL1 mice. (a and b) Plasma VLDL clearance without (a) and after heparin injection (b) in LpL1 and hLpL^GPI/LpL1 mice. [³H]palmitate-labeled VLDL produced in LpL1 mice was intravenously injected into LpL1 and hLpL^GPI/LpL1 male mice, and plasma was obtained by retro-orbital bleeds. The plasma count at 0.5 min after injection was considered as the injected dose. Plasma VLDL clearance did not differ between LpL1 (open circles, n = 9) and hLpL^GPI/LpL1 mice (filled circles, n = 8). For FCR, LpL1 versus hLpL^GPI/LpL1 = 13.7 ± 6.6 versus 12.1 ± 4.4 pools/h. Heparinized hLpL^GPI/LpL1 mice (filled circles, n = 10) had faster clearance of radiolabeled VLDL than LpL1 mice (open circles, n = 9). For FCR, LpL1 versus hLpL^GPI/LpL1 15.3 ± 6.0 versus 24.3 ± 7.4 pools/h, P < 0.02. (c and d) Heart uptake of VLDL-TG (c) and after heparin (d) from LpL1 and hLpL^GPI/LpL1 mice. (c) Hearts of hLpL^GPI/LpL1 (black bar, n = 8) mice had 54% more VLDL-TG uptake than control (LpL1) hearts (white bar, n = 9). LpL1 versus hLpL^GPI/LpL1 = 0.016 ± 0.004 versus 0.025 ± 0.009 (heart dpm/injected dpm). (d) In the presence of heparin, hLpL^GPI/LpL1 mice (black bar, n = 10) had 26% more VLDL-TG uptake in the hearts compared with control LpL1 mice (white bar, n = 9). LpL1 versus hLpL^GPI/LpL1 = 0.015 ± 0.002 versus 0.019 ± 0.003 (heart dpm/injected dpm). Values are expressed as means ± SD. *P < 0.05; **P < 0.01.